Department of biochemistry and Molecular biology
Deepak Bastia, PhD
Biochemistry and Molecular Biology
Gaylord and Dorothy Donnelley Chair in Biomedical Sciences
1985-2001 Professor, Duke University Medical Center, Durham, NC
1979-1985 Associate Professor, Duke University Medical Center, Durham, NC
1977-1979 Assistant Professor, UAB, Birmingham, AL
1974-1977 Postdoctoral Fellow, Yale University, New Haven, CT
1972-1974 Postdoctoral Fellow, University of Colorado, Boulder, CO
1971-1972 Postdoctoral Fellow, Princeton University, Princeton, NJ
1971 Ph.D., University of Chicago, Chicago, IL
1965 M.S., IARI, New Delhi, India
Office (DDB-313): 843-792-0491
Lab (DDB-320): 843-876-2244
The primary focus of our laboratory is on the investigations of the molecular mechanisms of replication fork arrest, genome stability, checkpoint controls using Saccharomyces cerevisiae and Schizosaccharomyces pombe as model systems. These are topics of intense current interest not only from the perspectives of eukaryotic DNA transactions but also of Cancer Biology. Our laboratory is also interested in the molecular analysis of the human “timeless” protein and TIPIN (timeless-interacting protein).
Molecular Mechanisms of Cellular Aging. Recently, using yeast as a model system, we have worked out one (the major) pathway of cellular aging. We have shown that two molecular mechanisms act in a sequence to control replicative life span: (i) autoinhibition of a replication terminator protein called Fob1 and chromosome kissing. Further work is underway to study the molecular mechanisms that lead to aging and senescence.
Our laboratory offers outstanding training in nucleic acids biochemistry, enzymology of DNA replication and on protein nucleic acids and protein-protein interactions.
Recent Publications | Additional Publications
Bastia D, Srivastava P, Zaman S, Choudhury M, Mohanty BK, Bacal J, Langston LD, Pasero P, O'Donnell ME. (2016) Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest. Proc Natl Acad Sci USA, Epub June 13, 2016
Jaiswal R, Choudhury M, Zaman S, Singh S, Santosh V, Bastia D, Escalante CR. (2016) Functional architecture of the Reb1-Ter complex of Schizosaccharomyces pombe. Proc Natl Acad Sci USA 113(16):E2267-76.
Zaman S, Choudhury M, Jiang JC, Srivastava P, Mohanty BK, Danielson C, Humphrey SJ, Jazwinski SM, Bastia D. (2016) Mechanism of Regulation of Intrachromatid Recombination and Long-Range Chromosome Interactions in Saccharomyces cerevisiae. Mol Cell Biol 36(10):1451-63.
Choudhury M, Zaman S, Jiang JC, Jazwinski SM, Bastia D. (2015) Mechanism of regulation of 'chromosome kissing' induced by Fob1 and its physiological significance. Genes Dev 29(11):1188-201. PMID: 26063576
Jaiswal R, Singh SK, Bastia D, Escalante CR. (2015) Crystallization and preliminary X-ray characterization of the eukaryotic replication terminator Reb1-Ter DNA complex. Acta Crystallogr F Struct Biol Commun 71(Pt 4):414-8. PMID: 25849502
Bastia D, Zaman S. (2014) Mechanism and physiological significance of programmed replication termination. Semin Cell Dev Biol 30:165-73. Review. PMID: 24811316
Bairwa NK, Mohanty BK, Stamenova R, Curcio MJ, Bastia D. (2011) The intra-S phase checkpoint protein Tof1 collaborates with the helicase Rrm3 and the F-box protein Dia2 to maintain genome stability in Saccharomyces cerevisiae. J Biol Chem 286(4):2445-54. PMID: 21087929
Bastia D and Singh S. (2011) Chromosome kissing and modulation of replication termination. Bioarchitect 1(1):24-28. PMID: 21866258
Singh S, Sabatinos S, Forsburg S, Bastia D. (2010) Regulation of replication termination by Reb1 protein-mediated action at a distance. Cell 142:868-878.
Bairwa NK, Zzaman S, Mohanty BK, Bastia D. (2010) Replication fork arrest and rDNA silencing are two independent and separable functions of the replication terminator protein Fob1 of Saccharomyces cerevisiae. J Biol Chem 285(17):12612-9. PMID: 20179323
Saxena M, Singh S, Zzaman S, Bastia D. (2010) Investigations of pi initiator protein-mediated interaction between replication origins alpha and gamma of the plasmid R6K. J Biol Chem 285(8):5695-704. PMID: 20029091
Mohanty BK, Bairwa NK, Bastia D. (2009) Contrasting roles of checkpoint proteins as recombination modulators at Fob1-Ter complexes with or without fork arrest. Eukaryot Cell 8(4):487-95. PMID: 19234097.
Bastia D, Zzaman S, Krings G, Saxena M, Peng X, Greenberg MM. (2008) Replication termination mechanism as revealed by Tus-mediated polar arrest of a sliding helicase. Proc Natl Acad Sci USA 105:12831-12836.
Biswas S and Bastia D. (2008) Mechanistic insights into replication termination as revealed by investigations of the Reb1-Ter3 complex of S. pombe. Mol Cell Biol 28:6844-6857.
Swan M, Bastia D, Davies C. (2006) Crystal structure of pi initiator protein-iteron complex of plasmid R6K: implications for initiation of plasmid DNA replication. Proc Natl Acad Sci USA 103:18481-18486.
Krings G and Bastia D. (2006) Molecular architecture of a eukaryotic DNA replication terminus-terminator protein complex. Mol Cell Biol 26:8061-8074.