Department of biochemistry and Molecular biology

Mirko Hennig, PhD

Associate ProfessorMirko Hennig, PhD

2006-2010      Assistant Professor – Dept of Biochemistry & Molecular Biology, MUSC
2002-2006      Assistant Professor – Dept of Molecular Biology, The Scripps Research
                          Institute, La Jolla, CA.
2001-2002     Senior Research Associate – Dept of Molecular Biology, The Scripps Research
                         Institute, La Jolla, CA.
1999-2001     Research Associate – Dept of Molecular Biology, The Scripps Research
                         Institute, La Jolla, CA

Education
1999      Ph.D. – Johann-Wolfgang-Goethe University of Frankfurt/M, Germany.

Contact Info
Email: hennig@musc.edu
Office: 843-876-2382
Lab: 843-792-7918
Fax: 843-792-4322
DDB-213

Research Interests

It is the goal of our research program to understand the essential features of specific, high affinity RNA recognition by proteins and small molecules. Experimentally, NMR spectroscopy combined with a wide range of biochemical and biophysical methods are used to characterize biological targets such as retroviral mRNA pseudoknots involved in ribosomal –1 frameshifting and the regulatory Rev-RRE mRNA-protein complex, important for HIV replication. Methodological developments to facilitate investigations of these macromolecular complexes by NMR spectroscopy represent another key element of our research.

Recent Publications

Roth BM, Ishimaru D, and Hennig M. The core Microprocessor component DiGeorge syndrome critical region 8 (DGCR8) is a non-specific RNA-binding protein. J Biol Chem. 2013 Jul 26. [Epub ahead of print]

Casu F, Duggan BM, and Hennig M. The arginine-rich RNA-binding motif of HIV-1 Rev is intrinsically disordered and folds upon RRE binding. Biophys J. 2013; 105:1004-1017.

Roth BM, Ishimaru D, Hennig M. The core Microprocessor component DiGeorge syndrome critical region 8 (DGCR8) is a non-specific RNA-binding protein. J Biol Chem. 2013 Jul 26. [Epub ahead of print] PMID: 23893406 [PubMed - as supplied by publisher].

Ishimaru D, Plant EP, Sims AC, Yount BL Jr, Roth BM, Eldho NV, Pérez-Alvarado GC, Armbruster DW, Baric RS, Dinman JD, Taylor DR, Hennig M. RNA dimerization plays a role in ribosomal frameshifting of the SARS coronavirus. Nucleic Acids Res. 2013 Feb 1;41(4):2594-2608. Epub 2012 Dec 28.

Roth BM, Hennig M. Backbone (1)H (N), (13)C, and (15)N resonance assignments of the tandem RNA-binding domains of human DGCR8. Biomol NMR Assign. 2012 Jul 3. [Epub ahead of print] PubMed PMID: 22752847.

Parnham S, Gaines WA, Duggan BM, Marcotte WR Jr, Hennig M. NMR assignments of the N-terminal domain of Nephila clavipes spidroin 1. Biomol NMR Assign. 2011Oct;5(2):131-3. Epub 2010 Dec 10. PubMed PMID: 21152998; PubMed Central PMCID: PMC3090696.

Marenchino M, Armbruster DW, Hennig M. Rapid and efficient purification of RNA-binding proteins: application to HIV-1 Rev. Protein Expr Purif. 2009 Feb;63(2):112-9. Epub 2008 Sep 25. PubMed PMID: 18852051; PubMed Central PMCID: PMC2624574.

Carlomagno T, Amata I, Williamson JR, Hennig M. NMR assignments of HIV-2 TAR RNA. Biomol NMR Assign. 2008 Dec;2(2):167-9. Epub 2008 Oct 12. PubMed PMID: 19636896.

Rhome R, McQuiston T, Kechichian T, Bielawska A, Hennig M, Drago M, Morace G, Luberto C, Del Poeta M. Biosynthesis and immunogenicity of glucosylceramide in Cryptococcus neoformans and other human pathogens. Eukaryot Cell. 2007 Oct;6(10):1715-26. Epub 2007 Aug 10. Review. PubMed PMID: 17693597; PubMed Central PMCID: PMC2043385.

Cornish PV, Giedroc DP, and Hennig M. (2006) Dissecting Non-Canonical Interactions in Frameshift stimulating mRNA Pseudoknots. J. Biomol. NMR 35: 209-223.  PubMed PMID: 16865417.

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