Department of biochemistry and Molecular biology
Mirko Hennig, PhD
2006-2010 Assistant Professor – Dept of Biochemistry & Molecular Biology, MUSC
2002-2006 Assistant Professor – Dept of Molecular Biology, The Scripps Research
Institute, La Jolla, CA.
2001-2002 Senior Research Associate – Dept of Molecular Biology, The Scripps Research
Institute, La Jolla, CA.
1999-2001 Research Associate – Dept of Molecular Biology, The Scripps Research
Institute, La Jolla, CA
1999 Ph.D. – Johann-Wolfgang-Goethe University of Frankfurt/M, Germany.
It is the goal of our research program to understand the essential features of specific, high affinity RNA recognition by proteins and small molecules. Experimentally, NMR spectroscopy combined with a wide range of biochemical and biophysical methods are used to characterize biological targets such as retroviral mRNA pseudoknots involved in ribosomal –1 frameshifting and the regulatory Rev-RRE mRNA-protein complex, important for HIV replication. Methodological developments to facilitate investigations of these macromolecular complexes by NMR spectroscopy represent another key element of our research.
Casu F, Duggan BM, and Hennig M. The arginine-rich RNA-binding motif of HIV-1 Rev is intrinsically disordered and folds upon RRE binding. Biophys J. 2013; 105:1004-1017.
Roth BM, Ishimaru D, Hennig M. The core Microprocessor component DiGeorge syndrome critical region 8 (DGCR8) is a non-specific RNA-binding protein. J Biol Chem. 2013 Jul 26. [Epub ahead of print] PMID: 23893406 [PubMed - as supplied by publisher].
Roth BM, Hennig M. Backbone (1)H (N), (13)C, and (15)N resonance assignments of the tandem RNA-binding domains of human DGCR8. Biomol NMR Assign. 2012 Jul 3. [Epub ahead of print] PubMed PMID: 22752847.
Parnham S, Gaines WA, Duggan BM, Marcotte WR Jr, Hennig M. NMR assignments of the N-terminal domain of Nephila clavipes spidroin 1. Biomol NMR Assign. 2011Oct;5(2):131-3. Epub 2010 Dec 10. PubMed PMID: 21152998; PubMed Central PMCID: PMC3090696.
Marenchino M, Armbruster DW, Hennig M. Rapid and efficient purification of RNA-binding proteins: application to HIV-1 Rev. Protein Expr Purif. 2009 Feb;63(2):112-9. Epub 2008 Sep 25. PubMed PMID: 18852051; PubMed Central PMCID: PMC2624574.
Carlomagno T, Amata I, Williamson JR, Hennig M. NMR assignments of HIV-2 TAR RNA. Biomol NMR Assign. 2008 Dec;2(2):167-9. Epub 2008 Oct 12. PubMed PMID: 19636896.
Rhome R, McQuiston T, Kechichian T, Bielawska A, Hennig M, Drago M, Morace G, Luberto C, Del Poeta M. Biosynthesis and immunogenicity of glucosylceramide in Cryptococcus neoformans and other human pathogens. Eukaryot Cell. 2007 Oct;6(10):1715-26. Epub 2007 Aug 10. Review. PubMed PMID: 17693597; PubMed Central PMCID: PMC2043385.
Cornish PV, Giedroc DP, and Hennig M. (2006) Dissecting Non-Canonical Interactions in Frameshift stimulating mRNA Pseudoknots. J. Biomol. NMR 35: 209-223. PubMed PMID: 16865417.