Department of biochemistry and Molecular biology
Bidyut Mohanty, PhD
Research Assistant Professor
Biochemistry and Molecular Biology
1991-2001 Postdoctoral Research, Duke University Medical Center, Durham, NC
1987 Ph.D., Indian Agricultural Research Institute, India
1982 M.S., Utkal University, India
1979 B.S., Utkal University, India
BSB-529 and BSB 505
Genome stability in yeast.
(I) Genome Integrity: Cells are constantly exposed to various genotoxins that can cause DNA replication stress and affect genome integrity. However, cells employ various means to respond to insults by the genotoxins. Checkpoints, DNA replication machinery and DNA repair systems maintain genome integrity. Data from budding yeast show that various other pathways also respond to genotoxin exposure. We are studying cross-talks between the DNA integrity pathway proteins and proteins of other pathways such as sphingolipid pathway, actin and actin regulators in the maintenance of genome integrity and cell survival.
(II) Recombination and aging: Programmed replication arrest in yeast ribosomal DNA has been shown to induce recombination that plays a key role yeast lifespan. Our interest is to dissect the recombination pathways that controls yeast lifespan.
Tripathi K, Mor V, Bairwa NK, Del Poeta M, and Mohanty BK. (2012) Hydroxyurea treatment inhibits proliferation of Cryptococcus neoformans in mice. Frontiers in Microbiology: Fungi and their interactions. 3:187. doi: 10.3389/fmicb.2012.00187 (corresponding author).
Bastia D, Zzaman S, and Mohanty BK. (2012) Site-directed mutagenesis and yeast reverse 2 hybrid-guided selections to investigate the mechanism of replication termination. InTech - open science (Invited Review), in press.
Bairwa NK, Mohanty BK, Stamenova R, Curcio MJ, and Bastia D. (2011) The intra-S phase checkpoint protein Tof1 collaborates with the helicase Rrm3 and the F-box protein Dia2 to maintain genome stability in Saccharomyces cerevisiae. J Biol Chem. 286:2445-2454. PMID:21087929.
Tripathi K, Matmati N, Zheng WJ, Hannun YA, and Mohanty BK. (2011) Cellular morphogenesis under stress is influenced by the sphingolipid pathway gene ISC1 and DNA integrity checkpoint genes in Saccharomyces cerevisiae. Genetics 189:533-547. PMID:21840863.