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Center for Cell Death, Injury and Regeneration

Center Microscopes

Zeiss LSM 510 NLO in Drug Discovery
BD Biosciences CARV II in Drug Discovery
Zeiss LSM 510 in Hollings Cancer Center
Leica TSC SP2 AOBS in Hollings Cancer Center
Olympus FV 10i LIV in Drug Discovery
Olympus FV 1200 MPE in Drug Discovery

In addition computer workstations are available for image analysis with

  • NIH Image J
  • Adobe Photoshop
  • Zeiss Image Examiner
  • IP Lab
  • Olympus Viewer
  • Metamorph

Zeiss LSM 510 NLO Laser Scanning Confocal Microscope with Multiphoton Excitation
(Instructions on using microscope) 

      Capabilities

  • Confocal fluorescence imaging using single and multiphoton laser excitation.
  • Multiphoton fluorescence imaging in both descanned and non descanned modes.
  • Brightfield and DIC imaging using single and multiphoton excitation.
  • Spectral detection using META detector.
  • Other imaging modes, including time-lapse, multi-track, intravital, depth, ratio, PLIM (phosphorescence lifetime imaging), FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer).

     Laser Sources

  • 458, 477, 488 and 514-nm Argon laser
  • 543-nm He-Ne laser
  • 633-nm He-Ne laser
  • Ti-Sapphire multiphoton chameleon laser tunable from 690 to 1040 nm

      Description

The microscope has a fluorescent mercury arc and tungsten halogen lamps for visual specimen inspection and focusing. The system is housed in a dark room and mounted on a vibration isolation table. The microscope is equipped with a variety of objective lenses, dichroics and filters for imaging different fluorescent probes. In order to perform live cell imaging, the microscope is equipped with temperature and CO2 controlled environmental chamber with options for both open and closed perfusion imaging. The system is fully automated and computer controlled. An Eppendorf microinjection system is also available for use.

      Location

          Room DD521E in the Drug Discovery Building.

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BD Biosciences CARV II for Video Rate Confocal Imaging

(Instruction on using microscope)

       Capabilities

  • Confocal fluorescence imaging at video rates ("real time") using single photon 
    excitation 
  • Wide field fluorescence imaging 
  • Bright field and DIC imaging 
  • Time-lapse, ratio, 3-dimensional deconvolution, intravital, FRAP and FRET

        Description
   
          The CARV II spinning disc confocal microscope provides capability for video 
          rate 
confocal microscopy. The spinning disk unit is paired with an inverted Zeiss 
          Axiovert  200 M 
microscope and uses panchromatic fluorescence  excitation from 
          a metal halide lamp for confocal imaging.

        Location

          Room DD526 in the Drug Disocvery Building. 

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Zeiss 510 META Laser Scanning Confocal Microscope
(
Instruction on using microscope)



    Capabilities

  • Confocal fluorescence imaging using single photon laser excitation.
  • Brightfield and DIC imaging using single photon excitation.
  • Spectral detection using META detector.
  • Other imaging modes, including time-lapse, multi-track, intravital, depth, ratio, FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer).

     Laser Sources

  • 458, 477, 488 and 514-nm Argon laser
  • 543-nm He-Ne laser
  • 633-nm He-Ne laser

      Description

The microscope has a fluorescent mercury arc and tungsten halogen lamps for visual specimen inspection and focusing. The system is housed in a dark room and mounted on a vibration isolation table. The microscope is equipped with a variety of objective lenses, dichroics and filters for imaging different fluorescent probes. In order to perform live cell imaging, the microscope is equipped with temperature and CO2 controlled environmental chamber with options for both open and closed perfusion imaging. The system is fully automated and computer controlled. 

      Location

          Room 355 in the Hollings Cancer Center.

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Leica TCS SP2 AOBS Laser Scanning Confocal Microscope 
(Instruction on using microscope)



    Capabilities

  • Confocal fluorescence imaging using single photon laser excitation.
  • Brightfield and DIC imaging using single photon excitation.
  • Filter-free programmable acousto-optical beam splitter for dynamic beam splitting
  • Spectral detection by high efficiency SP prism spectrophotometer
  • Other imaging modes, including time-lapse, multi-track, intravital, depth, ratio, FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer).

     Laser Sources

  • 458, 477, 488 and 514-nm Argon laser
  • 543-nm He-Ne laser
  • 633-nm He-Ne laser

      Description

The microscope has a fluorescent mercury arc and tungsten halogen lamps for visual specimen inspection and focusing. The system is housed in a dark room and mounted on a vibration isolation table. The microscope is equipped with a variety of objective lenses, dichroics and filters for imaging different fluorescent probes. The system is fully automated and computer controlled. 

      Location

          Room HO704 in the Hollings Cancer Center.


Olympis FV10i LIV laser scanning confocal microscope
(Instructions on using microscope) 

      Capabilities

  • Confocal fluorescence imaging using single photon laser excitation.
  • Phase contrast imaging using single photon excitation.
  • Other imaging modes, including time-lapse, multi-track, and depth

     Laser Sources

  • 405-nm diode laser
  • 473-nm diode laser
  • 559-nm diode laser
  • 635-nm diode laser

      Description

The Fluoview FV10i is a self-contained confocal laser scanning microscope with desirable features for live-cell imaging, including compact design, vibration isolation environmental control and a light-tight cover, eliminating the need for a dark room. The FV10i has the same standard functionality of a larger and more expensive confocal laser scanning microscopes with easy-to-use software. The system is equipped with four lasers, which means a wide variety of fluorophores can be imaged. Detection side is with a variable bandpass filter allowing selection of the most suitable wavelength for a particular fluorophore being imaged. The system is equipped with a 10X 0.4 NA dry lens and a 60X 1.2 NA water-immersion lens. Specimen holders are usable for 35mm glass bottom dishes, glass slides, and cover glass chambers.

      Location

          Room DD521E in the Drug Discovery Building.

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Olympus FV1200 MPE Intravital Microscope
(Instructions on using microscope)

    

Capabilities

  • Multiphoton fluorescence imaging with GaAsP external detectors.
  • Second harmonic imaging.
  • Long working distance silicone-oil and water-immersion objectives

     Laser Sources

  • Ti-Sapphire multiphoton chameleon laser tunable from 690 to 1040 nm

      Description

The microscope is designed for intravital imaging of live animals. It is equipped with sensitive GaAsP detectors for improved sensitivity. Further it has long working distance silicone-oil and water-immerison objectives for deeper imaging.

      Location

          Room DD521D in the Drug Discovery Building.

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