Center Microscopes Zeiss LSM 510 NLO in Quadrangle
Olympus FV1000 MPE in Quadrangle (Demonstration unit on loaner from Olympus Inc.)
BD Biosciences CARV II in Quandrangle
Zeiss LSM 510 in Strom Thurmond
Leica TSC SP2 AOBS in Hollings Cancer Center Zeiss LSM 510 NLO Laser Scanning Confocal Microscope with Multiphoton Excitation
(Instructions on using microscope) 
Capabilities - Confocal fluorescence imaging using single and multiphoton laser excitation.
- Multiphoton fluorescence imaging in both descanned and non descanned modes.
- Brightfield and DIC imaging using single and multiphoton excitation.
- Spectral detection using META detector.
- Other imaging modes, including time-lapse, multi-track, intravital, depth, ratio, PLIM (phosphorescence lifetime imaging), FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer).
Laser Sources - 458, 477, 488 and 514-nm Argon laser
- 543-nm He-Ne laser
- 633-nm He-Ne laser
- Ti-Sapphire multiphoton chameleon laser tunable from 690 to 1040 nm
Description The microscope has a fluorescent mercury arc and tungsten halogen lamps for visual specimen inspection and focusing. The system is housed in a dark room and mounted on a vibration isolation table. The microscope is equipped with a variety of objective lenses, dichroics and filters for imaging different fluorescent probes. In order to perform live cell imaging, the microscope is equipped with temperature and CO controlled environmental chamber with options for both open and closed perfusion imaging. The system is fully automated and computer controlled. An Eppendorf microinjection system is also available for use.
Location Room QE3C2 in the Quandrangle Building. Back to top
Olympus FV1000 MPE Laser Scanning Confocal Microscope with Multiphoton Excitation
Capabilities - Confocal fluorescence imaging using single and multiphoton laser excitation.
- Multiphoton fluorescence imaging in both descanned and non descanned modes.
- Brightfield and DIC imaging using single and multiphoton excitation.
- Spectral detection.
- Other imaging modes, including time-lapse, multi-track, intravital, depth, ratio, PLIM (phosphorescence lifetime imaging), FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer).
Laser Sources - 488 and 514-nm Argon laser
- 405-nm Diode laser
- 440-nm Diode laser
- 559-nm Diode laser
- 635-nm Diode laser
- Ti-Sapphire multiphoton Mai Tai laser tunable from 690 to 1040 nm
Description The microscope has a fluorescent mercury arc and tungsten halogen lamps for visual specimen inspection and focusing. The system is housed in a dark room and mounted on a vibration isolation table. The microscope is equipped with a variety of objective lenses, dichroics and filters for imaging different fluorescent probes. In order to perform live cell imaging, the microscope is equipped with temperature and CO2 controlled environmental chamber with options for both open and closed perfusion imaging. The system is fully automated and computer controlled. The system has a SIM scanner for simultaneous laser stimulation and image acquisition. Variable barrier filters allow acquisition wavelengths to be set freely to suit various fluorochromes.
Location
Room QF414 in the Quandrangle Building. Back to top
BD Biosciences CARV II for Video Rate Confocal Imaging
(Instruction on using microscope) 
Capabilities - Confocal fluorescence imaging at video rates ("real time") using single photon
excitation - Wide field fluorescence imaging
- Bright field and DIC imaging
- Time-lapse, ratio, 3-dimensional deconvolution, intravital, FRAP and FRET
Description
The CARV II spinning disc confocal microscope provides capability for video
rate confocal microscopy. The spinning disk unit is paired with an inverted Zeiss
Axiovert 200 M microscope and uses panchromatic fluorescence excitation from
a metal halide lamp for confocal imaging. Location Room QF 418 in the Quandrangle Building. Back to top
Zeiss 510 META Laser Scanning Confocal Microscope
(Instruction on using microscope)
Capabilities
- Confocal fluorescence imaging using single photon laser excitation.
- Brightfield and DIC imaging using single photon excitation.
- Spectral detection using META detector.
- Other imaging modes, including time-lapse, multi-track, intravital, depth, ratio, FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer).
Laser Sources - 458, 477, 488 and 514-nm Argon laser
- 543-nm He-Ne laser
- 633-nm He-Ne laser
Description The microscope has a fluorescent mercury arc and tungsten halogen lamps for visual specimen inspection and focusing. The system is housed in a dark room and mounted on a vibration isolation table. The microscope is equipped with a variety of objective lenses, dichroics and filters for imaging different fluorescent probes. In order to perform live cell imaging, the microscope is equipped with temperature and CO controlled environmental chamber with options for both open and closed perfusion imaging. The system is fully automated and computer controlled.
Location Room 647 in the Strom Thurmond Building. Back to Top
Leica TCS SP2 AOBS Laser Scanning Confocal Microscope
(Instruction on using microscope) 
Capabilities
- Confocal fluorescence imaging using single photon laser excitation.
- Brightfield and DIC imaging using single photon excitation.
- Filter-free programmable acousto-optical beam splitter for dynamic beam splitting
- Spectral detection by high efficiency SP prism spectrophotometer
- Other imaging modes, including time-lapse, multi-track, intravital, depth, ratio, FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer).
Laser Sources - 458, 477, 488 and 514-nm Argon laser
- 543-nm He-Ne laser
- 633-nm He-Ne laser
Description The microscope has a fluorescent mercury arc and tungsten halogen lamps for visual specimen inspection and focusing. The system is housed in a dark room and mounted on a vibration isolation table. The microscope is equipped with a variety of objective lenses, dichroics and filters for imaging different fluorescent probes. The system is fully automated and computer controlled.
Location Room HO704 in the Hollings Cancer Center. Back to Top |
