Ischemia/Reperfusion injury in the liver:
Hepatic ischemia/reperfusion injury develops after liver transplantation, removal of hepatic tumors, circulation shock and acute exposure to toxic substances. Our lab focuses on the cellular and molecular mechanisms of ischemia reperfusion injury in the liver. Specifically we are applying new techniques of confocal microscope to investigate the role of mitochondrial permeability tranisition (MPT) in the pathogensis of liver injury. Figures 1 show images of liver cells after ischemia (left panel) and reperfusion (right panel). The dark voids in the left panel show the mitochondria which exclude the green fluorescing calcein indicating healthy mitochondria. However upon reperfusion the dark voids dissapear (right panel) and get filled in by the calcein indicating mitohondrial permeability transition (MPT).
Figure 1. Calcein fluorescene in hepatocytes under 4 h of ischemia (left panel)
and reperfuion (20 min post reperfusion). The dark voids on the left image indicates
ability of the mitochondria to exclude the calcein indicating healthy mitochondria. The
right image after reperfusion indicate the onset of MPT by the fact that the dark mitochondria
voids fill up with calcein.