Medical University | MUSC Hospital | Directory | A-Z Index

Center for Oral Health Research

MUSC logo

left

 COHR: Center for Oral Health Research | Oral Histology & Digital Imaging Core | Laser Capture Microdissection

Laser Capture Microdissection

The state-of-the-art technology of laser capture microdissection (LCM) provides researchers with the ability to accurately analyze DNA, RNA and protein from pure populations of cells, such as tumor cells that are captured from complex heterogeneous tissue samples. Protocols have been developed and optimized for acquisition of high-quality RNA and DNA from both frozen and formalin-fixed, paraffin-embedded tissue. Care is taken to preserve the integrity of the samples at all times, ensuring high quality retrieval of molecular targets.

Services available:

  • Laser Capture Microdissection of pure populations of cells
  • DNA/RNA/protein extraction and QA/QC from microdissected samples
  • RNA/cDNA Amplification from microdissected, frozen, and FFPE tissues
  • Support for grant applications by providing LCM budgets and support letters

The LCM procedure is remarkably simple and robust. The method is extraordinarily gentle and ideal for microdissection of single cells or small numbers of cells. The LCM system utilizes a laser microbeam that melts a thermoplastic membrane that sticks to the selected cells, which can then be lifted and secured in a microfuge tube containing the appropriate extraction solutions. The transferred tissue on the film retains its original morphology, thereby allowing microscopic verification of the specificity of the captured material. Using this strategy, a single small cluster up to thousands of cells can be procured safely and without contamination. The Agilent 2100 Bioanalyzer and Nanodrop ND1000 monitor the quality and quantity of extracted RNA, DNA, as well as proteins from the LCM sample.

Contact:

Kimberlee Lisicki, MA
Research Specialist
BSB 129
792-5122
lisicki@musc.edu

Courtney J. Haycraft, PhD
L-COHR Director

BSB 230D
792-8095
haycraft@musc.edu


 

Scholars Day