Oral Preclinical Research Models
The Laboratory Core (L-COHR) within the Center for Oral Health Research is dedicated to providing MUSC researchers with the equipment, techniques, and technical expertise necessary to study all aspects of oral health and disease. L-COHR services are available to researchers at MUSC and other institutions. The facility is equipped with state of the art equipment and experienced staff to assist both new and experienced researchers. To access our services, please visit our iLab website (musc.ilabsolutions.com)
Researchers are encouraged to consult with L-COHR staff prior to sample preparation. Staff are available for:
•Assistance with sample preparation, processing and analysis
•Experimental design consultation
•Training in specialized techniques
Animal Models Available:
- Xenograft tumor model
This tumor model is created by injection of human head and neck cancer cell lines into the floor of the mouth or flanks of athymic nude mice
- 4NQO oral cancer model
Mice develop oral neoplasia or esophageal tumors with prolonged exposure to 4-nitroquinoline-1 oxide (4NQO)-containing drinking water. In addition to allowing investigators to study inflammatory cytokine responses,the 4NQO model allows investigators to study tumorogenesis and progression in genetically altered mouse strains.
- Periodontitis model
A mouse model of periodontitis is generated by injecting LPS from an oral pathogen into the periodontium leading to loss of alveolar bone over time.
Services available to researchers include:
SubQ, IP, or floor-of-mouth injection of cell lines
Animal health monitoring
Oral injection of LPS
Imaging of tumor progression (in vivo)
Assistance with dissection and tissue collection
Murine Oral Cancer Tissue Array and Tumor Samples:
L-COHR has generated tissue microarrays from various stages of oral cancer lesions in mice exposed to 4NQO. All samples have been scored by a pathologist and the complete array contains a 54 tissue samples in a 6x9 arrangement including 18 invasive squamous cell carcinomas, 24 epithelial hyperplasia or dysplasia lesions, and 12 control tissues. In addition, portions of many of these tumors were preserved in RNAlater solution to allow isolation of RNA or protein for downstream analyses. These tissues are available to researchers upon request.
|Charlye Marger, MS|
|Hong Yu, MD|
|Courtney J. Haycraft, PhD|