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 Marine Biomedicine and Environmental Sciences | Faculty | Demetri D. Spyropoulos, Ph.D.

Demetri D. Spyropoulos, Ph.D.

Research Interest:
I have 22 years experience in the manipulation of embryonic stem cells (ESCs) and the generation and analysis of genetically engineered mouse models. More recently, we have developed an efficient procedure for the cryopreservation and retrieval of viable cell types from lung tissue of stranded (deceased) aquatic mammals. Using these cryopreserved samples, we have also generated induced pluripotent stem cells (iPSCs) from lung fibroblasts through the activation of 4 stem cell factors. Technical challenges associated with the marine source material aguably support our proposal for the seamless application of this novel procedure for the cryopreservation of viable human patient lung tissues in next-generation biobanking. These cells are being used to bioengineer reconstructed lungs and thereby study the basic biology behind stem/differentiated cell adaptation to oxidative stress. Although oxidative stress response has been extensively studied in embryonic stem cell (ESC) models of terrestrial mammals, parallels have not been investigated in marine mammals. To fill this gap, I propose studies comparing oxidative stress between PSW iPSCs and mouse ESCs at the Medical University of South Carolina (MUSC) that use novel technologies demonstrate that mouse ESC protein expression significantly differs in hypoxic vs. normoxic conditions, setting a precedent for further investigation and human therapies.

Selected Publications:

1.  Higuchi T, Bartel FO, Masuya M, Deguchi T, Henderson KW, Li R and Spyropoulos DD. Thymomegaly, Microsplenia, and Defective Homeostatic Proliferation of Peripheral Lymphocytes in p51-Ets1 Isoform-Specific Null Mice. Molecular and Cellular Biology. 2007;27(9):3353-66. PMCID: PMC1899970.

2.  Spyropoulos DD, Pharr PN, Lavenburg KR, Jackers P, Papas TS, Ogawa M, et al. Hemorrhage, Impaired Hematopoiesis, and Lethality in Mouse Embryos Carrying a Targeted Disruption of the Fli1 Transcription Factor. Molecular and Cellular Biology. 2000;20(15):5643-52. PMCID: PMC86032.

3.  Spyropoulos DD, Bartel FO, Higuchi T, Deguchi T, Ogawa M, Watson DK. Marker-assisted study of genetic background and gene-targeted locus modifiers in lymphopoietic phenotypes. Anticancer Res. 2003;23(3A):2015-26. PMID: 12894574

4.  Bartel FO, Higuchi T, Spyropoulos DD. Mouse models in the study of the Ets family of transcription factors. Oncogene. 2000;19(55):6443-54. PMID: 11175360

5.  Kawada H, Ito T, Pharr PN, Spyropoulos DD, Watson DK, Ogawa M. Defective megakaryopoiesis and abnormal erythroid development in Fli-1 gene-targeted mice. Int J Hematol. 2001;73(4):463-8.

Demetri D. Spyropolous, Ph.D.

Demetri D. Spyropoulos, Ph.D.
Associate Professor
Medical University of South Carolina

Ph.D., Harvard University

Dr. Spyropoulos's Contact Information

Hollings Marine Lab
221 Ft Johnson Rd.
Charleston, SC 29412

Current MBES Student and Postdoctoral Fellow
- Emily Allen