SC COBRE in Oxidants, Redox Balance, and Stress Signaling
Cell and Molecular Imaging Core
The COBRE Imaging Core houses five major microscope systems: 1) a Zeiss LSM 510 NLO laser scanning confocal/multiphoton microscope equipped with a Choherent Chameleon tunable femtosecond Ti-Sapphire laser and META spectral detection, 2) a BD Biosciences CARV II real-time spinning disk confocal microscope equipped with a Photometrics Cascade 1K Digital camera and IPLab image processing and analysis software and 3) a Zeiss LSM 510 laser scanning confocal microscope and META spectral detection, 4)Leica AOBS TCS SP2 laser scanning confocal microscope 5) Olympus Fluoview FV10i laser scanning desktop microscope. The two Zeiss microscopes and the FV10i are equipped with environmental chambers for temperature and gas phase control to allow high resolution non-destructive 3-dimensional imaging of living cells, tissues, small animal organs and organisms. Major applications of this instrumentation include 1) live cell imaging of parameter-sensitive fluorophores to monitor ions, electrical potentials, oxygen and nitrogen radical generation, pyridine nucleotide reduction, mitochondrial and plasmalemmal membrane permeability, cell viability (apoptosis and necrosis), fluorescent protein labeling and other parameters; 2) high resolution imaging of tissue sections for immunocytochemistry and green fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET) to characterize and quantify interactions between specific molecules; and 4) intravital microscopy to monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation, gene expression and other factors in living animals. Ancillary equipment includes tissue culture hoods and incubators for specimen preparation; and fluorescence and absorbance plate readers for parallel measurements in cultured cells grown on multi-well plates. Imaging core services will promote the success of the individual COBRE projects and also provide training and assistance to junior investigators studying oxidative stress and stress signaling related to the overall theme of this COBRE. Currently the imaging core is supporting 1. Dr. Sherine Chan’s project of looking at mtDNA mutations and 2. James Chou’s projects looking at mitochondrial morphology and function in leukemia and nueroprotection models and development of novel inhibitors for mitochondrial protection.
John Lemasters, Ph.D.
Professor of Pharmaceutical and Biomedical Sciences & Professor of Biochemistry
GlaxoSmithKline Distinguished Endowed Chair in Advanced Cellular Technologies
Venkat Ramshesh, Ph.D.
Research Instructor of Pharmaceutical and Biomedical Sciences