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SC COBRE in Oxidants, Redox Balance and Stress Signaling

Cores

Cell and Molecular Imaging Core

The Cell and Molecular Imaging Core provides major support in confocal and multiphoton microscopy to COBRE investigators.

Mass Spectrometry Core

The Mass Spectrometry Core will promote the success of the individual COBRE projects and provide training and assistance to junior investigators studying oxidative stress and stress signaling.  We are working with COBRE funded investigators to 1) enrich and identify glutathionylated peptides and oxidized cysteine residues, 2) measure differentially expressed proteins, 3) evaluate enzyme active site conformation by photoaffinity cross-linking, and 4) identify differentially acetylated proteins.

Metabolomics Core

The Metabolomics Core provides the technology and expertise for the identification and quantification of low molecular weight metabolites (typically <1500 Da). The Metabolomics Core is under the direction of Dr. Craig Beeson, a bioanalytical and organic chemist with wide ranging experience in fluorescence/NMR spectroscopy and mass spectrometry as applied to metabolic flux analyses. 

Analytical Redox Biology Core

The specific aims of the ARB Core are: 1) Provide ROS /RNS identification and quantification using state-of-the-art techniques;  2) Perform quantitative analysis of ROS/RNS (redox molecules and metabolites), including those associated with calcium mobilization and changes in energy metabolism; and 3) Provide expertise and technology for in depth biochemical analysis of thiol-centered enzyme activities and define protein:protein interactions.