SC COBRE in Oxidants, Redox Balance, and Stress Signaling
The primary focus of the core facility is the characterization of metabolites related to cellular redox and primary energy metabolism – the source of both the primary and secondary cellular redox species. The facility provides access to traditional, ‘gold standard’ techniques such as isotopomer, radiometric, and spectroscopic analyses. The core is also a development site for the Seahorse Biosciences fluorometric biosensor technology used to measure metabolic fluxes (i.e., oxygen consumption, CO2 and lactate extrusion) in real time using multiwell plates. The basic Seahorse applications enable high throughput metabolic measurements with small sample sizes that have transformed quantitative analyses of metabolic fluxes. Innovative adaptations of the technologies developed in the core facility are providing access to real time flux measurements of redox species in cells and tissues. The immediate goal has been to coordinate these recent strategic technological acquisitions into a core that provides analytical support for the efforts of the COBRE investigators and their collaborators. As part of these efforts we have developed and validated an extracellular flux assay of bioenergetics metabolism to support the studies of mtDNA mutations in Sherine Chan’s lab. We have also screened a library of HDAC inhibitors against a cell-based model for mitochondrial dysfunction in retinal degeneration for James Chou’s lab. Finally, we are spearheading the development of a flux-based assay for the pentose phosphate pathway in collaboration with researchers in the Danyelle Townsend lab.
Craig Beeson, Ph.D.
Associate Professor of Pharmaceutical and Biomedical Sciences