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MUSC Proteomics Center

MUSC Proteomics Center

Overview

The MUSC Proteomics Center is focused on clinical and translational proteomics and development of centralized diagnostics.  It is comprised of independent investigators, two core laboratories (Mass Spectrometry Facility and MS Imaging Research Center), and supported by external funding, as well as the MUSC Provost Office, College of Medicine, Hollings Cancer Center, NIH-COBRE and SmartState funding. Mass spectrometry is the key analytical method for proteomics research, and the Center has recently obtained significant upgrades to an extensive mass spectrometry facility. Current instrumentation includes three ion trap instruments (one Thermo Orbitrap Elite with ETD, two Thermo LTQ's) for analysis of post-translational modifications like phosphorylation and glycosylation. Quantitative proteomics of cultured cells using SILAC and iTRAQ/TMT labeling, as well as characterization of multiple types of post-translational modifications, is the primary emphasis for use of the Orbitrap. Also in the facility are a dual-source Bruker Solarix 7T Fourier Transform-ICR mass spectrometer and two Autoflex MALDI-TOF instruments used primarily for MALDI tissue imaging.  The MALDI tissue imaging approaches are histopathology directed, and used for two-dimensional spatial profiling of proteins, lipids, glycans, metabolites and small molecule drugs. The Center leverages high-performance computing resources with modern protein search algorithms, mass spectrometry based quantification analysis, statistical analysis (frequentist or Bayesian techniques), data-driven systems biology, -omic integration techniques and classifier development (machine learning based) to generate actionable and hypothesis driving results.

For information about quantitative proteomic strategies contact Dr. Lauren Ball (ballle@musc.edu).

Further information about The Mass Spectrometry Facility and its services can be obtained from Jennifer Bethard (bethard@musc.edu) or at the Mass Spectrometry Facility web site.

For information about mass spectrometry imaging capabilities in the MS Imaging Research Center, contact Dr. Peggi Angel (angelp@musc.edu) or Dr. Richard Drake (draker@musc.edu).

For information about exosomes obtained from clinical specimens and N-linked glycosylation profiling, contact Dr. Richard Drake (draker@musc.edu).

For information about computational proteomics and bioinformatics approaches contact Dr. Benjamin Neely (neely@musc.edu).

Additional proteomic mass spectrometry capabilities are described for the MUSC Nephrology Proteomics Laboratory, Director, Michael Janech (janechmg@musc.edu) at their webpage.

Key Personnel

Director, MUSC Proteomics Center

Richard R. Drake, PhD
843-792-4505
draker@musc.edu

Director, Mass Spectrometry Facilty

Lauren E. Ball, PhD
843-792-4513
ballle@musc.edu

Director, MSC Imaging Research Center

Peggi Angel, PhD
843-792-8410
angelp@musc.edu

Director, Computational Proteomics and Bioinformatics

Benjamin A. Neely, PhD
843-792-9946
neely@musc.edu

Lab Manager, Mass Spectrometry Facility

Jennifer Bethard, MS
843-792-8637
bethard@musc.edu

 

 
 
 

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