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Office of Research Integrity

Recommended Rodent Anesthesia & Analgesia

an attempt to simplify the protocol process, the IACUC has approved a list of recommended anesthetic and analgesic agents for rodents. These are recommendations and alternate agents will be permitted as long as their use is justified in the protocol.

It is acceptable to list more than one potential anesthetic regimen, but each proposed regimen must be complete (i.e. Surgical plane of anesthesia will be established with ketamine/xylazine 80-100/10 mg/kg i.p. OR metomidate/fentanyl 60/.06 mg/kg s.c.).

If you have any questions about the listed agents, please contact one of the DLAR veterinarians for assistance. All drugs marked (*) are controlled substances and required a Controlled Substances License in the name of the PI.

Analgesics (mice & rats) | Anesthetics for Mice | Anesthetics for Rats

DLAR Recommended Analgesics for Rodents
DrugMouse dose (mg/kg)Rat dose (mg/kg)
Buprenorphine*0.05-0.1 s.c., q 12 hrs0.01-0.05 s.c. or i.v., q 8-12 hrs
0.1-0.25 p.o., q 8-12 hrs
Meperidine*10-20 s.c. or i.m., q 2-3 hrs10-20 s.c. or i.m., q 2-3 hrs
Local anesthesia:
Lidocaine/Bupivacaine
Lidocaine/BupivacaineLidocaine/Bupivacaine
Acetominophen200 p.o.200 p.o.
Carprofen2.5-5.0 s.c., q 24 hrs2.5-5.0 s.c., q 24 hrs
Meloxicam1.0-10.0, i.p.1-2 s.c., q 24 hrs

Severe pain may be better addressed by the addition of a NSAID to an opioid. This multi-model approach allows for action at different points on the pain pathways and will allow for a lower dosage of both components. Buprenorphine, alone, is recommended for only moderate pain management.

DLAR recommends that analgesics be administered preemptively; i.e., before a painful stimulus.



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DLAR Recommended Anesthetics for Mice
DrugDose (mg/kg) & RouteEffectDuration of Anesthesia (min)Sleep Time (min)
Isoflurane4-5% for induction; 1.5-3% for maintenanceSurgical anesthesiacontinuous during deliveryrecovery begins shortly after cessation
Ketamine*/acepromazine100/5 i.p.Immobilization/ anesthesia20-3040-120
Ketamine*/acepromazine/xylazine100/2.5/2.5 i.p.Surgical anesthesia20-3060-120
Ketamine*/xylazine/acepromazine100/20/3 i.p.Surgical anesthesia45-60130-145
Ketamine*/diazepam*100/5 i.p.Immobilization/ anesthesia20-3060-120
Ketamine*/medetomidine75/1.0 i.p.Surgical anesthesia (minor procedures)20-3060-120
Ketamine*/midazolam*100/5 i.p.Immobilization/ anesthesia20-3060-120
Ketamine*/xylazine80-100/10 i.p.Surgical anesthesia20-3060-120
Methohexital*10 i.v.Surgical anesthesia510
Metomidate/fentanyl*60/.06 s.c.Surgical anesthesia40-6090-120
Pentobarbital*40-70 i.p.Immobilization/ anesthesia20-40120-180
Thiopental*25-50 i.v.; i.p.Surgical anesthesia5-1010-15

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DLAR Recommended Anesthetics for Rats
DrugDose (mg/kg) & RouteEffectDuration of Anesthesia (min)Sleep Time (min)
Isoflurane4-5% for induction; 1.5-3% for maintenanceSurgical anesthesiacontinuous during deliveryrecovery begins shortly after cessation
Ketamine*/acepromazine75/2.5 i.p.Light anesthesia20-30120
Ketamine*/diazepam*75/5 i.p.Light anesthesia20-30120
Ketamine*/medetomidine75/.5 i.p.Surgical anesthesia20-30120-240
Ketamine*/midazolam*75/5 i.p.Light anesthesia20-30120
Ketamine*/xylazine75-100/10 i.p.Surgical anesthesia20-30120-240
Methohexital*10-15 i.v.Surgical anesthesia510
Pentobarbital*40-50 i.p.Light anesthesia15-60120-240
Thiopental*30 i.v.Surgical anesthesia1015

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