Office of Research Integrity

Animal Use & Housing Outside DLAR Facilities

Definitions | Policy | Requirements

Definitions

  • Animal Housing: Any location where live vertebrate animals are maintained 24 hours or more.
  • Animal Use: Any location where live vertebrate animals are handled or kept at any time.
  • DLAR Facilities: All locations where animals are used or housed under the direct supervision of DLAR staff on a daily basis.
  • Husbandry Log: Record of all routine care provided to animals on a daily basis, like feeding, watering, health check, and environmental monitoring.
  • Justification: Scientific reason for keeping animals outside of DLAR Facilities for 12 hours or longer.
  • Outside DLAR: Any location of animal use or housing which is not directly under the supervision of DLAR staff, like PI labs or spaces shared among multiple PIs in a workgroup or department.
  • PI-Maintained Colony: Any location of animal housing outside DLAR where animals are kept >72 hours.

Policy

The MUSC IACUC recognizes housing inside DLAR facilities to be the optimal animal husbandry environment. It is also acknowledged that there may be valid reasons for animals to be outside DLAR facilities for extended periods of time. Justification is required when live vertebrate animals will be kept outside of DLAR facilities for periods of 12 hours or longer. In addition to this justification, protocols in which live warm-blooded vertebrates will be housed 24 hours or longer outside DLAR facilities will only be reviewed via Full Committee Review at a convened meeting.

PI-maintained colonies outside DLAR space refer specifically to all colonies that reside outside DLAR for >72 hours. Husbandry and animal monitoring are performed solely by research personnel. PI-maintained rodent colonies must submit a Rodent Husbandry Checklist and husbandry log for protocol approval. For other species, such as reptiles, amphibians and fish, a husbandry SOP and log must be submitted as part of the animal protocol for IACUC approval. All animals, regardless of species, must be monitored daily, including weekends and holidays. Documentation of daily monitoring is required and will be monitored during IACUC semiannual inspections. (03/11)

Requirements

  • Use of Animals Outside DLAR:
    • Complete section in Vertebrate Animal Review Application (VARA) on Location of Research, or submit Amendment to Protocol with "Change in Location" selected.
      • Include all specific rooms outside DLAR where animals will be handled or kept at any time, even if only for euthanasia.
      • Briefly describe activities related to live animals in each location (i.e. survival surgery, catheter placement, euthanasia & tissue collection, gavage, imaging, etc.).
      • Provide justification if animals will be kept in any non-DLAR location for 12 hours or longer.
      • Indicate whether rodent survival surgery will be performed in any listed location.
        • Complete all applicable appendices.
        • IACUC inspection of all new surgical sites must be obtained prior to first surgery.
    • Submit an Amendment to Protocol request prior to use of animals in any new location. Locations no longer in use for animal work may be removed via amendment request.
    • All use locations will be inspected at least semiannually by the IACUC and are expected to be clean and tidy with all appropriate supplies used for live animals properly maintained and accessible.
    • All animal housing and use locations are subject to additional scheduled or unannounced inspections by the IACUC, DLAR and other authorized internal and external regulatory authorities.
  • Housing of Animals Outside DLAR:
    • Additional IACUC review of protocol is required and approval for housing warm-blooded vertebrates 24 hours or longer may only be granted after Full Committee Review at a convened meeting.
  • PI-Maintained Colonies:
    • Appendix 7A "Rodent Husbandry Checklist", or Custom Husbandry SOP (non-rodents only) must be completed as part of IACUC application.
    • Husbandry Log must be included for all species.
      • A standard log for rodents is available on the IACUC Forms page, but custom logs may be approved as long as all required information is included.
    • IACUC inspection of all new PI-maintained colony sites must be obtained prior to housing of animals.
    • All animals must be monitored daily for food, water, health, and environmental conditions. This includes holidays and weekends.
    • All monitoring documentation must be maintained in the animal housing area and available upon request to IACUC, DLAR or Compliance personnel.
    • Animal health or care problems must be reported to DLAR promptly. Emergency care is available by contacting the DLAR Veterinarian or Vet Tech on duty.