Office of Research Integrity
It has been determined that multiple survival surgery for collection of ovarian tissue is a common procedure in research for amphibians, such as Xenopus sp. Any amphibian undergoing these surgical procedures will have them conducted under anesthesia using aseptic technique. A single animal may undergo a maximum of three procedures with the third surgical procedure being terminal. The animal should have a minimum recovery time of two months before harvesting the ovary from the other side. These protocols do not require full committee review by the IACUC.
Surgical procedures - Some labs are approved for rodent surgical procedures outside of DLAR facilities. These labs should be using aseptic technique, have records of adequate anesthesia and postoperative analgesia and records of surgical results. NIH guidelines require use of sterile gloves, sterile instruments, glass bead sterilization of instruments between procedures, aseptic preparation of the incision site, lab records of the procedure performed, and removal of sutures or staples 10-14 days postoperatively. Unanticipated complication rates of >10% must be reported to the IACUC. Veterinary staff must be consulted to treat any postoperative complications. Analgesics must be administered to all animals which may experience pain due to the procedure. Potentially painful procedures are those which would be expected to cause pain in humans. Exceptions to these policies must be approved in advance by the IACUC and must be scientifically justified as causing problems with the research goals. (05/09)