2006-2008 Ph.D. Chonbuk National University, Jeonju, Republic of Korea
2008-2011 Post-doctoral Fellow, Chonbuk National University, Jeonju, Republic of Korea
2011-2012 Post-doctoral Fellow, University of Florida, Gainesville, FL
2002-2008 Assistant of Pharmacology, Chonbuk National University, Jeonju, Republic of Korea
2010 Research Assistant Professor of Pharmacology, Chonbuk National University, Jeonju, Republic of Korea
The Wang lab focuses on pancreatic islet cell biology and transplantation immunology in the treatment of type 1 and type 2 diabetes and chronic pancreatitis.
Type 1 diabetes: Islet cell transplantation is the most potent therapy for patients with type 1 diabetes. However, there are two major problems besetting this process. First, there are not enough islets available for transplant. Second, islets after transplantation are often undergoing apoptosis due to the stresses encountered during islet harvest and after transplantation and the consequent immune rejection response, thus their function are compromised. Ongoing projects in the Wang lab focus on solving these problems. Major areas of investigation include:
(i) The roles of a protective gene, heme oxygenase-1 (HO-1) and its products, carbon monoxide and bilirubin in islet transplantation;
(ii) Generation of insulin-secreting cells from adult stem cells (e.g., adipose stem cells, umbilical cord stem cells) to expand source of transplanted islets;
(iii) Developing novel encapsulation methods using FDA approved nanoparticles to protect islets from apoptosis and immune rejection;
(iv) Understanding the role of the Regulator of G Protein Signaling 2 (RGS2) in islet regeneration.
Chronic Pancreatitis (CP): CP is a long-standinginflammationof thepancreasthat alters its normal structure and functions. Current therapies for CP patients focus on pain relief medically, endoscopically and surgically (by resection of diseased parenchyma and drainage of obstructed ducts). In patients with intractable pain and those with diffuse small duct diseases, total pancreatectomy with islet autotransplantation (TP-IAT) can be an ideal treatment option. Compared to other treatment options for CP, TP-IAT has a higher potential to eliminate pancreatic pain without total sacrifice of the endocrine function of the pancreas. Together with Drs. David B Adams and Katherine A Morgan, we are developing interventional procedures including induction of protective genes, nano-encapsulation, and co-transplantation with mesenchymal stem cells to improve islet yield quantitiy and quality in order to prevent onset of surgical diabetes after TP-IAT in patients with chronic pancreatitis.
Type 2 diabetes: Prevalence of obesity has become a social and economic burden for health care today. In addition, obesity is a major cause for insulin resistance and type 2 diabetes (T2D). We have found that HO-1 induction or bilirubin administration reduce hyperglycemia, increases insulin sensitivity in both the leptin-receptor deficient (db/db) mice and the diet-induced obese mice. The goal of our study is to further understand the molecular mechanisms of HO-1 and bilirubin, and to develop clinical applicable therapies to improve obesity and insulin resistance.
2.Kim DS, Li B, Rhew KY, Oh HW, Lim HD, Lee W, Chae HJ, Kim HR. The regulatory mechanism of 4-PBA against ER-stress induced cell death and autophagy in human gingival fibroblasts. Arch Pharm Res. 2012 Jul;35(7):1269-78. Epub 2012 Aug 3.
5. Bhandary B, Piao CS, Kim DS, Lee GH, Chae SW, Kim HR, Chae HJ. The protective effect of rutin against ischemia/reperfusion-associated hemodynamic alteration through antioxidant activity. Arch Pharm Res. 2012 Jul;35(7):1269-78. Epub 2012 Aug 3.
6. Marahatta A, Kim DS, Kim HK, Kim HR, Chae HJ. Isolation of Tanshinone IIA and cryptotanshinone in salvia miltiorrhiza using two conventional extraction techniques and quantification by validated HPLC method. Int J Pharm Pharm Sci. Vol4, Suppl1, 627-631.
27. Chae HJ, Lee GY, Yang SK, Kim DS, Yun KJ, Kim EC, Kim HM, Chae SW, Kim HR. Effect of high molecular weight water-soluble chitosan on the trabecular bone and thickness in ovariectomized rats. Immunopharmacol Immunotoxicol. 2007;29(3-4):439-49.
28. Kim DS, Jeong SK, Kim HR, Kim DS, Chae SW, Chae HJ.Effects of triglyceride on ER stress and insulin resistance.Biochem Biophys Res Commun. 2007 Nov 9;363(1):140-5. Epub 2007 Sep 4
29. Lee GH, Kim HK, Chae SW, Kim DS, Ha KC, Cuddy M, Kress C, Reed JC, Kim HR, Chae HJ. Bax inhibitor-1 regulates endoplasmic reticulum stress-associated reactive oxygen species and heme oxygenase-1 expression. J Biol Chem. 2007 Jul 27;282(30):21618-28. Epub 2007 May 24.
30.Kim DS, Woo ER, Chae SW, Ha KC, Lee GH, Hong ST, Kwon DY, Kim MS, Jung YK, Kim HM, Kim HK, Kim HR, Chae HJ. Plantainoside D protects adriamycin-induced apoptosis in H9c2 cardiac muscle cells via the inhibition of ROS generation and NF-kappaB activation. Life Sci. 2007 Jan 2;80(4):314-23. Epub 2006 Sep 26.
31. Kim DS, Kim HR, Woo ER, Kwon DY, Kim MS, Chae SW, Chae HJ. Protective effect of calceolarioside on adriamycin-induced cardiomyocyte toxicity. Eur J Pharmacol. 2006 Jul 10;541(1-2):24-32. Epub 2006 May 9.
32. Chae HJ, Ha KC, Kim DS, Cheung GS, Kwak YG, Kim HM, Kim YM, Pae HO, Chung HT, Chae SW, Kim HR. Catalase protects cardiomyocytes via its inhibition of nitric oxide synthesis. Nitric Oxide. 2006 May;14(3):189-99. Epub 2006 Jan 5.
33.Kim DS, Kim HR, Woo ER, Hong ST, Chae HJ, Chae SW. Inhibitory effects of rosmarinic acid on adriamycin-induced apoptosis in H9c2 cardiac muscle cells by inhibiting reactive oxygen species and the activations of c-Jun N-terminal kinase and extracellular signal-regulated kinase. Biochem Pharmacol. 2005 Oct 1;70(7):1066-78.
34. Chae HJ, Kim HR, Kim DS, Woo ER, Cho YG, Chae SW. Saeng-Ji-Hwang has a protective effect on adriamycin-induced cytotoxicity in cardiac muscle cells. Life Sci. 2005 Mar 18;76(18):2027-42.
35. Chae HJ, Yang SK, Kim DS, Kim HM, Chae SW, Keum KS, Kim HR. Ge-Jee-Bok-Ryung-Hwan induces apoptosis in human cervical carcinoma HeLa cells--an endoplasmic reticulum stress pathway--. Life Sci. 2004 Nov 5;75(25):2997-3016.