This page is intended as a guide for researchers setting up Biosafety Level 2 (BSL2) laboratories. Adherence to this guide will result in compliance with the CDC's guidelines (Biosafety in Microbiological and Biomedical Laboratories, ed. 5) and facilitate passing a lab inspection conducted by the Biosafety Officer. Facility Requirements - Lockable doors
- Impermeable work surfaces
- Sealed/locked windows
- Access to an autoclave
| - Hand washing station (sink, soap/alcohol gel and paper towels)
- Eyewash
- Load bearing furniture (not covered in absorbent material)
- Negative air pressure (can be confirmed by the Biosafety Officer)
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Supplies - Autoclave tray
- Biohazard waste container/bags
- Dust pan and broom
- Disinfectants (e.g. bleach and ethanol)
- Sharps container
| - BSL-2 placards (Provided by Biosafety Officer)
- Secondary container for transporting biohazards (sealed, leak proof and labeled as biohazard)
- Disposable gloves
- Lab coats
- Face and respiratory protection may be needed based on procedures and agents
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Equipment and Supplies for Mammalian/Viral Culture - Certified biosafety cabinet
- Vacuum trap for liquid waste
- HEPA filtered vacuum line
- Method for decontaminating pipets
- *Recommended* Designated cloth or disposable lab coats for viral culture
- *Recommended* Centrifuges with sealed rotors or safety cups to contain aerosols
- Examples with ordering information
Procedures - Personnel should follow the lab's safety protocol.
- All liquid waste should be inactivated prior to disposal. Bleach should be added to a final concentration of 10% and allowed 30 minutes to inactivate infectious agents.
- Solid waste should be decontaminated with 10% bleach and/or autoclaved prior to disposal.
Coming Soon: Guidance for Research Involving | - Human materials and blood borne pathogens
- Biological safety cabinets (Tissue culture hoods)
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